Detection: Glycosaminoglycans By PAGE & Silver Staining l Protocol Preview

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  • Published on:  Friday, August 12, 2022
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    Detection of Glycosaminoglycans by Polyacrylamide Gel Electrophoresis and Silver Staining - a 2 minute Preview of the Experimental Protocol

    Wells B. LaRiviere, Xiaorui Han, Kaori Oshima, Sarah A. McMurtry, Robert J. Linhardt, Eric P. Schmidt
    University of Colorado Anschutz Medical Campus, Department of Medicine; University of Colorado Anschutz Medical Campus, Medical Scientist Training Program; Rensselaer Polytechnic Institute, Department of Chemistry and Chemical Biology; Curtin University, Department of Health Sciences; Denver Health Medical Center, Department of Medicine;

    This report describes techniques to isolate and purify sulfated glycosaminoglycans (GAGs) from biological samples and a polyacrylamide gel electrophoresis approach to approximate their size. GAGs contribute to tissue structure and influence signaling processes via electrostatic interaction with proteins. GAG polymer length contributes to their binding affinity for cognate ligands.

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